Escherichia coli (Migula) Castellani and Chalmers
53323 ™
Product category Bacteria Classification Enterobacteriaceae, Escherichia
Strain designation JM109
Type strain No
Applications Molecular biology
Product format Frozen
Storage conditions -80°C or colder
Specific applications transformation host
Preceptrol No
Genotype F' traD36 proA+ proB+ lacIq delta(lacZ)M15 delta(lac-proAB) supE44 hsdR17 recA1 gyrA96 thi-1 endA1 relA1 e14- lambda- Markers (rk- mk+)
Comments Bacteriophage host This is a host for M13 and other filamentous bacteriophages Technical information ATCC Technical Services does not have technical information on patent deposits that are not produced or characterized by ATCC.
Additional information can be found in the corresponding patent available from the patent holder or with the U.S. and/or international patent office.
Medium ATCC Medium 2664: M9 Minimal Agar, Modified
Temperature 37°C
Atmosphere Aerobic
Handling procedure Open thawed vial according to enclosed instructions or visit www.atcc.org for instructions.
Aseptically transfer the entire contents to a 5-6 mL tube of #2664 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary tubes.
Use several drops of the primary broth tube to inoculate a #2664 plate and/or #2664 agar slant.
Incubate at 37°C for 24 hours.
Handling notes A host strain for M13 and other filamentous bacteriophage. - Cell 46: 645-652, 1986 .patent
Deposited as Escherichia coli (Migula) Castellani and Chalmers Depositors Crop Genetics International N.V. Patent depository This material was deposited with the ATCC Patent Depository to fulfill U.S. or international patent requirements.
This material may not have been produced or characterized by ATCC.
As an International Depository Authority (IDA) for patent deposits, ATCC is required to complete viability testing only at time of initial deposit of patent material. Patent deposits are made available on behalf of the Depositor when the pertinent U.S. or international patent is issued, but material may not be used to infringe the patent claims. Patent number 4,889,809
ATCC53323
