EO771
CRL-3461 ™
EO771 is an epithelial-like cell that was isolated from the mammary gland of a patient with carcinoma. This cell line was deposited by R Anderson, Translational Breast Cancer Research Program, Olivia Newton-John Cancer Research Institute.
Product categoryAnimal cells
OrganismMus musculus, mouse
Morphologyepithelial-likeTissueBreast; Mammary glandDiseaseCarcinoma; Breast
Applications3D cell culture
Product formatFrozenStorage conditionsVapor phase of liquid nitrogen
Specific applicationsThis cell line can be used to analyze genes that regulate metastasis of breast cancer and/or drugs that might reduce spontaneous metastasis in immune competent C57Bl/6 mice.
Cells per vialApproximately 2.0 to 3.0 x 106Volume1.0 mLGrowth propertiesAdherentCommentsThe cells display an elongated morphology. Depositor note: cells are genetically unstable in continuous culture. The EO771 mammary tumor was first reported in 1948 as a spontaneous mammary carcinoma arising in a C57Bl/6 mouse.
Unpacking and storage instructionsCheck all containers for leakage or breakage.
Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use.
Complete mediumThe base medium for this cell line is Dulbecco's Modified Eagle's Medium (DMEM; ATCC 30-2002). To make the complete medium add the following components to the base medium:
10% Fetal Bovine Serum (FBS; ATCC 30-2020)
20 mM HEPES (Thermo Fisher Scientific cat# 15630-080)
Handling procedureTo ensure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C. Storage at -70°C will result in loss of viability.
Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Thawing should be rapid (approximately 2 minutes).
Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.
Transfer the vial contents to a centrifuge tube containing 9.0 mL complete culture medium. and spin at approximately 125 x g for 5 to 7 minutes.
Resuspend cell pellet with the recommended complete medium (see the specific batch information for the culture recommended dilution ratio). It is important to avoid excessive alkalinity of the medium during recovery of the cells. It is suggested that, prior to the addition of the vial contents, the culture vessel containing the complete growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6). pH (7.0 to 7.6).
Incubate the culture at 37°C in a suitable incubator. A 5% CO2 in air atmosphere is recommended if using the medium described on th
Bacterial and fungal testingNot detectedMycoplasma contaminationNot detectedVirus testingHepatitis B virus (HBV): Not detected
Cytomegalovirus (CMV): Not detected
Human Immunodeficiency virus (HIV): Not detected
Epstein-Barr virus (EBV): Not detected
Human papillomavirus (HPV): Not detected
