Colletotrichum gloeosporioides (Penzig) Penzig et Saccardo
20358 ™
An ampoule containing viable cells suspended in cryoprotectant.
Product category
Fungi
Classification
Fungi, Dikarya, Ascomycota, Pezizomycotina, Sordariomycetes, Hypocreomycetidae, Glomerellales, Glomerellaceae, Colletotrichum
Type strain
No
Isolation source
northern crownvetch, Aeschynomene virginica
Geographical isolation
United States; Arkansas
Applications
Biocontrol
Product format
Frozen
Storage conditions
-80°C or colder
General
Specific applications
biological control of northern jointvetch, Aeschynomene virginica
Preceptrol
No
Characteristics
Technical information
ATCC Technical Services does not have technical information on patent deposits that are not produced or characterized by ATCC. Additional information can be found in the corresponding patent available from the patent holder or with the U.S. and/or international patent office.
Handling information
Medium
ATCC Medium 322: Lima bean agar
Temperature
24°C
Handling procedure
Frozen ampoules packed in dry ice should either be thawed immediately or stored in liquid nitrogen. If liquid nitrogen storage facilities are not available, frozen ampoules may be stored at or below -70°C for approximately one week. Do not under any circumstance store frozen ampoules at refrigerator freezer temperatures (generally -20°C). Storage of frozen material at this temperature will result in the death of the culture.
To thaw a frozen ampoule, place in a 25°C to 30°C water bath, until just thawed (approximately 5 minutes). Immerse the ampoule just sufficient to cover the frozen material. Do not agitate the ampoule.
Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer at least 50 µL (or 2-3 agar cubes) of the content onto a plate or broth with medium recommended.
Incubate the inoculum/strain at the temperature and conditions recommended.
Inspect for growth of the inoculum/strain regularly for up to 4 weeks. The time necessary for significant growth will vary from strain to strain.
History
Legal disclaimers
ATCC20358
