Dengue virus type 3
VR-3380 ™
Dengue virus type 3 strain H87 is propagated in Vero cells (ATCC CCL-81). This culture was isolated from a patient sample in the Philippines.
Product category
Viruses
Product type
Animal virus
Classification
Flaviviridae, Flavivirus
Strain designation
H87
Isolation source
Presumed from serum from patient
Geographical isolation
Philippines
Applications
Infectious disease research
Vector-borne disease research
Zoonotic disease research
Product format
Frozen
Storage conditions
-70°C or colder
CommentsDerived by adaptation of ATCC VR-1256_FD to Vero cells (ATCC CCL-81). ATCC VR-1256_FD and ATCC VR-3380 have not been compared for sequence or infectivity in tissue culture.
Handling information
HostVero (ATCC CCL-81)
Effectscell rounding; cell sloughing; cell detachment; cell degeneration
Complete mediumEMEM (ATCC 30-2020) + 2% FBS (ATCC 30-2020)Temperature37°C
Incubation2-4 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 90% of the monolayer.Recommendation for infectionPlate cells 1-2 days days prior to infection and infect when cultures are 75-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.Key abbreviations°C, Degrees Celsius
CO2, Carbon dioxide
EMEM, Eagle's Minimum Essential Medium
FBS, Fetal bovine serum
MOI, Multiplicity of infection
