Neisseria meningitidis (Albrecht and Ghon) Murray
700110™
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Product category
Bacteria
Strain designation
CDC 522 [ET5 complex, Norwegian vaccine strain]
Type strain
No
Genome sequenced strain
Yes
Applications
Vaccine development
Product format
Freeze-dried
Storage conditions
2°C to 8°C
General
Specific applications
Used to produce vaccine
Preceptrol
No
Characteristics
Serotype
B:15:P1.7,16
Comments
Strains of the ET5 complex have caused meningococcal disease globally. This strain differs from the Cuban vaccine strain by outer membrane proteins.
Epidemiologically important
Handling information
Medium
ATCC Medium 814: GC Agar/Broth Medium
Temperature
37°C
Atmosphere
95% Air, 5% CO2
Handling procedure
Open vial according to enclosed instructions or visit www.atcc.org for instructions.
Rehydrate the entire pellet with approximately 0.5 mL of #814 broth. Aseptically transfer the entire contents to a 5-6 mL tube of #814 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary broth tubes.
Use several drops of the primary broth tube to inoculate a #814 plate and/or #814 agar slant.
Incubate at 37°C for 24-48 hours in an atmosphere of 5% CO2.
Handling notes
Brain Heart Infusion (Difco 0037) can be used to rehydrate vial contents if #814 broth is not available, but it will not support growth as well as the GC broth.
Additional information on this culture is available on the ATCC® web site at www.atcc.org.
Quality control specifications
Verification method
Whole-genome Sequencing
History
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