Dictyostelium discoideum Raper 拉丁名
ATCC 28368 編號(hào)
An ampoule containing viable cells (may include spores and mycelia) suspended in cryoprotectant.
Product category Fungi
Strain designation 別名 Ax-3
Type strain 模式菌株 No
Product format 提供形式 Freeze-dried
Storage conditions 保藏溫度 2°C to 8°C
Specific applications produces calmodulin transformation host hydrolysis of a phosphonate ester
Preceptrol No
Comments Actin binding to plasma membrane Thymidine kinase activity Part of the Eumycetozoan Project This strain may grow in axenic medium or with Klebsiella aerogens, but ATCC maintains this strain with a bacterial food source.
Medium 培養(yǎng)基 ATCC Medium 617: Bg-11 for marine blue-green algae
ATCC Medium 1034: Modified PYNFH medium (Available from ATCC as ATCC cat. no. 327-X)
Temperature 培養(yǎng)溫度 20-25°C
Atmosphere 需氧情況 Aerobic
Handling procedure For freeze-dry (lyophilized) ampoules: Open an ampoule according to enclosed instructions.
From a single test tube of sterile distilled water (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a sterile pipette and apply directly to the pellet. Stir to form a suspension.
Aseptically transfer the suspension back into the test tube of sterile distilled water. Let the test tube sit at room temperature (25°C) undisturbed for at least 2 hours; longer (e.g., overnight) rehydration might increase viability of some fungi. Mix the suspension well. Use 1.0 mL to inoculate recommended freshly-made and wetted solid medium and freshly-made liquid medium.
Agar plates should be overlaid with 2 mL of a liquid medium, either #671 or #1034 (with 10% fetal bovine serum included).
Include a control that receives no inoculum. Incubate the inoculum at the propagation conditions recommended. Inspect for growth of the inoculum/strain regularly.
The sign of viability is noticeable typically after 2-3 days of incubation.
However, the time necessary for significant growth will vary. Subculture from stationary liquid cultures after one week.
Sequenced data No DNA sequencing was performed in house on this product.
Deposited as Dictyostelium discoideum Raper Depositors WF Loomis
Chain of custody ATCC <-- WF Loomis
Special collection Eumycetozoan Project
Cross references 參考文獻(xiàn) GenBank D12883 gene coding for gp138 GenBank D12884 gene coding for gp138
ATCC28368
