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平臺(tái)編號(hào)：bio-132181
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國(guó)際編號(hào)：ATCC 19574
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拉丁屬名： Clostridium perfringens
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規(guī)格：freeze-dried
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用途：ATCC原裝進(jìn)口
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
Clostridium perfringens
Clostridium perfringens (Veillon and Zuber) Hauduroy et al. 拉丁名
ATCC 19574 編號(hào)
Toxinotype A 毒型A
Product category Bacteria Product type Anaerobe
Strain designation 別名 43F4
Type strain 模式菌株 No
Isolation 分離源 source Bile Geographical isolation United States; Maryland; Baltimore
Applications Agricultural research Enteric disease research Infectious disease research
Product format 提供形式 Freeze-dried
Storage conditions 保藏條件 2°C to 8°C
Specific applications Enteric Research
Preceptrol No
Genotype cpa, cpe, cpb2, pfoA Toxigenic Yes Toxin genes cpa (Alpha toxin) positive cpb2 (Beta2 toxin) positive cpe (Enterotoxin) positive etx (Epsilon toxin) negative pfoA (Perfringolysin O) positive
Comments Toxinotype A
Hydrolyzes bile acid conjugates The presence of cpa, cpe, cpb2, and pfoA genes was confirmed by PCR.
This strain does not produce epsilon toxin
Medium 培養(yǎng)基 ATCC Medium 2107: Modified Reinforced Clostridial
ATCC Medium 260: Trypticase soy agar/broth with defibrinated sheep blood
Temperature 培養(yǎng)溫度 37°C
Atmosphere 需氧情況 Anaerobic
Handling procedure Open vial according to enclosed instructions or visit www.atcc.org for instructions.
Under anaerobic conditions aseptically rehydrate the entire pellet with approximately 0.5 mL of #2107 broth.
Aseptically transfer the entire contents to a 5-6 mL tube of #2107 broth.
Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary broth tubes.
Best practice dictates the use of pre-reduced media. Use several drops of the primary broth tube to inoculate a #260 plate and/or #260 agar slant.
Incubate in an anaerobic atmosphere at 37°C for 24 hours. Incubate one agar plate aerobically at 37°C to check for contamination.
Deposited as Clostridium perfringens (Veillon and Zuber) Hauduroy et al. Depositors JF Reback Type of isolate Human
ATCC19574
