-
平臺編號：bio-117576
-
國際編號：ATCC 34873
-
拉丁屬名： Filobasidiella neoformans
-
規(guī)格：Freeze-dried
-
用途：ATCC原裝進(jìn)口
-
服務(wù)費用：
加載中…… - 訂購
-
注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
Filobasidiella neoformans
Filobasidiella neoformans Kwon-Chung, teleomorph 拉丁名
ATCC 34873 編號
An ampoule containing viable cells (e.g. yeast cells, spores) suspended in cryoprotectant.
Product category Fungi
Product type Yeast
Strain designation 別名 NIH B-3501 [CBS 6900, CBS 7697, CBS 7817, CCRC 20532]
Type strain 模式菌株 No
Genome sequenced strain Yes
Isolation 分離源 source Single-basidiospore isolate from a cross of ATCC 28957 x ATCC 28958.
Product format 提供形式 Freeze-dried
Storage conditions 保藏條件 2°C to 8°C
Specific applications Biomedical Research and Development Material assay of eumelanin pigments Ref Ref produces monophenol monooxygenase phenol oxidase, phenoloxidase Ref regulation of phenol oxidase and capsular polysaccharide Ref Preceptrol NoSerotype D Mating type alpha Genotype VNIV Morphology After 3 days on YM medium at 25°C, colony is cream-colored, mucoid. Cells are globose, single. No hyphae observed.
Comments Designated as type strain of Cryptococcus deneoformans Hagen et Boekhout by Hagen et al. (2015). Genome sequencing strain (The University of Oklahoma, USA). Smooth colony type Ref
Medium 培養(yǎng)基 ATCC Medium 28: Emmons' modification of Sabouraud's agar
ATCC Medium 200: YM agar or YM broth
ATCC Medium 1245: YEPD
Temperature 培養(yǎng)溫度 25-30°C
Atmosphere 需氧情況 Aerobic Handling procedure For freeze-dry (lyophilized) ampoules: Open an ampoule according to enclosed instructions.
From a single test tube of sterile distilled water (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a sterile pipette and apply directly to the pellet. Stir to form a suspension.
Aseptically transfer the suspension back into the test tube of sterile distilled water. Let the test tube sit at room temperature (25°C) undisturbed for at least 2 hours; longer (e.g., overnight) rehydration might increase viability of some fungi. Mix the suspension well.
Use several drops (or make dilutions if desired) to inoculate recommended solid or liquid medium. Include a control that receives no inoculum. Incubate the inoculum at the propagation conditions recommended. Inspect for growth of the inoculum/strain regularly.
The sign of viability is noticeable typically after 1-2 days of incubation. However, the time necessary for significant growth will vary from strain to strain.
Sequenced data 18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 26S ribosomal RNA gene, partial sequence GGTTTCCGTAGGTGAACCTGCGGAAGGATCAGTAGAGAATATTGGACTTTGGTCCATTTATCTACCCATCTACACCTGTGAACTGTTTATGTGCTTCGGC
ACGTTTTACACAAACTTCTAAATGTAATGAATGTAATCATATTATAACAATAATAAAACTTTCAACAACGGATCTCTTGGCTTCCACATCGATGAAGAACGCAGCGAAA
TGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAGTCTTTGAACGCAACTTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTTGAGAGTCATGAAAAT
CTCAATCCCTCGGGTTTTATTACCTGTTGGACTTGGATTTGGGTGTTTGCCGCGACCTGCAAAGGACGTCGGCTCGCCTTAAATGTGTTAGTGGGAAGGTGATTACCT
GTCAGCCCGGCGTAATAAGTTTCGCTGGGCCTATGGGGTAGTCTTCGGCTTGCTGATAACAACCATCTCTTTTTGTTTGACCTCAAATCAGGTAGGGCTACCCGCTGA
ACTTAAGCATATCAATAA D1D2 region of the 26S ribosomal RNA gene
ATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGTGAACCGGGAAGAGCTCAAATTTGAAATCTGGCGTCCTCCGGGCGTCCGAGTTG
TAATCTACAGAAACGTTTTCCGTGCTGGACCGTGTCTAAGTCCCTTGGAATAGGGTATCAAAGAGGGTGACAATCCCGTACTTGACACGATCACCAGTGCTCTGTGATA
CGTTTTCTACGAGTCGCGTTACTTGGGAGTGTAGCGCAAAATGGGTGGTAAACTCCATCTAAAGCTAAATATTGGTGGAAGACCGATAGCGAACAAGTACCGTGAGGGA
AAGATGAAAAGCACTTTGGAAAGAGAGTTAAACAGTACGTGAAATTGTTGAAAGGGAAACGATTGAAGTCAGTCGTGTCTATTGGGTTCAGCCAGTTCTGCTGGTGTAT
TCCCTTTAGACGGGTCAACATCAGTTCTGATCGGTGGATAAGGGCTGGAGGAATGTGGCACTCTTCGGGGTGTGTTATAGCCTCCTGTCGCATACACTGGTTGGGACTG
AGGAATGCAGCTCGCCTTTATGGCCGGGGTTCGCCCACGTTCGAGCTTAGGATGTTGACAAAATGGCTTTAAACGAC 26S ribosomal RNA gene and intergenic spacer (IGS), partial sequence
ATGGGAACGGGGTGCTGTAAGTGGTAGAGTAGCCTTGTTGCTACGATCCACTGAGGCTAAGCCCTTGTTCTATAGATTTGTCTCTAACATGTTGGGTCTCGGGGGGCTTCC
TCTAGAGACTTGGATGTAAGGGGCTTTACGCATTCACATAATATTAGTTGGGGACTGAGGGGGGTGTGTGCTGTGCGAGCCCGGCTGATAATTGTTTTAATTGTTAGCTTGG
ATTGCACACACAGTCTCATCAGTCTTCAGCTTGGGTTATCGATATTCTGTAAGTCCAAACTTATCCATTCACATTCACTGTAGTGTAGGTTGCAGAGAGGACTTGGAAAGTTCT
CTCTCTGCACTGGGCATACTACTGATTTTATGGTCATGGGGGACTTGGGAGCTGGTGCTTGTGTCGCATAAGGTAGAGTTGATGGTTGGACAGATAGATAAGCATTTTTATGT
CGCGGGGGACTTGGGGTAAGACGCCTTGCAGCAAGTAAGAGAGTCAAACAGTAGTTACTTGTTCTAGTCGTGGGGGACTTTGGTAGTGGTAGTGAGCCGGAATGAGAGATGG
ATAAATGCAGTATGGATGGTGACAGGAGGAGCAGCAGAGCGTATGCAGTGGATATGTATATAGTTGAGAAGGGATGGACAGTAGAACGTTTGAACGTTGAGGAATAATTGACC
CAAGGGTGATTCTAATAGACTTGTCAAGAAAAAGTTAAGTTGAATCAAGCTTGGTCAAGCAAAGTCTAGAAAAGATTTACAATCACCAAGAATTGCCCGAGTCGCGGGGGGCTT
GTCACTTCTCTTGCTCATTGTGGGTCCAGTCT glycerol-3-phosphate dehydrogenase 1 (GPD1) gene, partial cds
CAACGGTTTCGGTACGTAATCAATCCTTCTCTTCCCGTTGCTTCATATCCCACCGATGTGACTTCCAATATTGTGCGCTGGCTGTTCTTGAGTTATGGTAGCTAATAGCTATGTGA
TAGGTCGTATTGGTCGAATTGTTCTCAGGTATGCTTTCTTTAAGTCAACGGGGTAAGAAGCCCGTGTATCTAACGTTCAGGTAGGAACGCCATCGAGCATGGGGACCTCGAGGTT
GTTGCTGTCAACGAGTGAGTGTCCCAAAAGTGTTAATCCATTAGATGAATCACTTAATTGTCCATAGCCCTTTTATTGACTTAGACTACATGGTATGATACTTCTTTCCGCCCAATA
GCTTCGTACTGACTCTGTTGCGCAGGTCTACATGTTCAAGTATGACTCCGTAAGTCTCCCAAGTTCTAACGTTCTGTATGGCTTATTAACCTGATTTTCGACAGACACATGGTCGCT
TCAAGGGCTCCGTCGAGGTTAAGGACGGTAAACTTTACATCAACAACAAGGCCATTGCCGTCTTCGGCGAGAAAGACCCTGCCAACATCAAGTGGGG
History 來源歷史 Deposited as Cryptococcus neoformans (Sanfelice) Vuillemin Depositors KJ Kwon-Chung
Cross references 查詢文獻(xiàn) GenBank AF316889 Filobasidiella neoformans var. neoformans elongation factor 3 mRNA, complete cds.Intended use This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
ATCC34873
