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平臺編號：bio-114915
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國際編號：ATCC VR-1861*
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拉丁屬名： Bovine respiratory
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規(guī)格：Frozen
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用途：ATCC原裝進口
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服務費用：
加載中…… - 訂購
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注意事項：僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產品信息以出庫為準）
中文名稱暫無
Bovine respiratory syncytial virus (ATCC
VR-1861
)Classification Pneumoviridae, Orthopneumovirus
Agent Bovine respiratory syncytial virus
Strain A 51908
Applications Respiratory research
Biosafety Level 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format frozen
Storage Conditions -70°C or colderImages
CommentsATCC VR-1861 was derived from ATCC VR-794 following treatment with Mycoplasma Removal Agent (MRA) to remove Mycoplasma contamination.Effect on Host
CPE, Syncytia with cell sloughing/detachment
Recommended Host
BT cells (ATCC CRL-1390)
Growth Conditions
Temperature: 37°C
Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 70-80% confluent. Remove medium and inoculate with a small volume of virus (e.g., 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g., 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.
Incubation: 4-6 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80-90% of the monolayer.
Effect on Host
CPE, Syncytia with cell sloughing/detachment
Name of Depositor SB Mohanty
Source Trachea of calf with respiratory disease
References
Mohanty SB, et al. Experimentally induced respiratory syncytial viral infection in calves. Am. J. Vet. Res. 36: 417-419, 1975. PubMed: 164807
VR-1861)Classification Pneumoviridae, OrthopneumovirusAgent Bovine respiratory syncytial virus
Strain A 51908
Applications Respiratory research
Biosafety Level 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format frozen
Storage Conditions -70°C or colderImages
CommentsATCC VR-1861 was derived from ATCC VR-794 following treatment with Mycoplasma Removal Agent (MRA) to remove Mycoplasma contamination.Effect on HostCPE, Syncytia with cell sloughing/detachment
Recommended Host
BT cells (ATCC
CRL-1390)Growth Conditions
Temperature: 37°C
Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 70-80% confluent. Remove medium and inoculate with a small volume of virus (e.g., 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g., 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.
Incubation: 4-6 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80-90% of the monolayer.
Effect on Host
CPE, Syncytia with cell sloughing/detachment
Name of Depositor SB Mohanty
Source Trachea of calf with respiratory disease
References
Mohanty SB, et al. Experimentally induced respiratory syncytial viral infection in calves. Am. J. Vet. Res. 36: 417-419, 1975. PubMed: 164807
