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 Human respiratory syncytial virus 拉丁名
ATCC VR-1803 編號
Classification Paramyxoviridae, Pneumovirus
Agent Human respiratory syncytial virus
Strain ATCC-2012-11
Biosafety Level 安全等級 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 frozen
Storage Conditions -70°C or colderImages、CommentsThis item was isolated in cell culture by three passages in HEp-2 cells
This strain belongs to subtype BEffect on Host
CPE, refractile cell rounding and syncytia
Recommended Host 
HEp-2 (ATCC CCL-23)
Growth Conditions 生長條件
Temperature 培養(yǎng)溫度 : 37°C 
Recommendations for Infection: For best results, infection should be performed on a 70-80% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2), optimized for propagation (e.g. MOI 0.1-0.5 recommended). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 4-6 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
Effect on Host 
CPE, refractile cell rounding and syncytia
Name of Depositor ATCC
Source Specimen from a clinical patient in Virginia, 2012