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平臺編號：bio-091545
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國際編號：ATCC VR-682
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拉丁屬名： Caviid herpesvirus 2
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規(guī)格：frozen
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用途：ATCC原裝進口
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服務費用：
加載中…… - 訂購
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注意事項：僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產品信息以出庫為準）
Caviid herpesvirus 2
VR-682 編號
Product categoryViruses
Product typeAnimal virus
Strain designation 別名 22122
Isolation source
Submaxillary glands from a guinea pig.
Product format 提供形式 Frozen
Storage conditions 保藏溫度 -70°C or colder
Mycoplasma contamination Not detected
HostJH4 clone 1 (ATCC CCL-158)
Effectscell rounding; cell sloughing; CPE
Complete mediumF-12K (ATCC® 30-2004™) + 2% FBS (ATCC® 30-2020™)
Temperature 培養(yǎng)溫度 37°C
Atmosphere 95% Air, 5% CO2
Incubation 9-21 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% or greater of the monolayer.
Recommendation for infectionFor best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer.
Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation.
Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Handling notesBring total suspension to 10% FBS prior to freezing harvested virus.Key abbreviationsCPE, Cytopathic effect
E, Embryo
FBS, Fetal bovine serum
GP, Guinea pig
TC, Tissue culture
TCID[50], Median tissue culture infective dose
Volume 1.0 mL
Deposited asDeposited as Guinea pig salivary gland virusDepositorsMJ Collins, Jr., Microbiological Associates, Inc.Type of isolateAnimalCross references GenBank AB592928.1 Caviid herpesvirus 2 DNA, complete genome, strain: 22122
