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pGADT7-T培養(yǎng)條件 | LB,37℃,氨芐 |
啟動(dòng)子 | ADH1 |
復(fù)制子 | 2U |
質(zhì)粒分類 | 酵母系列,酵母雙雜交載體 |
質(zhì)粒大小 | 10.kb |
原核抗性 | Amp |
篩選標(biāo)記 | LEU2 |
克隆菌株 | DH5a |
培養(yǎng)條件 | 37度 |
表達(dá)宿主 | 酵母細(xì)胞 |
5'測(cè)序引物 | T7:TAATACGACTCACTATAGGG |
3'測(cè)序引物 | 根據(jù)序列設(shè)計(jì)引物 |
載體宿主 | 酵母菌 |
載體用途 | 雜交 |
原核抗性 | Amp |
真核抗性 | LEU2 |
質(zhì)粒簡(jiǎn)介 | pGADT7-T encodes a fusion of the SV40 large T-antigen (a.a. 86–708) and the GAL4 AD (a.a. 768–881). The SV40 large T DNA (GenBank Locus SV4CG) was derived from a plasmid referenced in Li & Fields (1993) and was cloned into pGADT7 using the EcoR I and Xho I sites. pGADT7-T has not been sequenced. |