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GeLu
    GeLu
  • 平臺編號:bio-68947
  • 國際編號:CCL-100?
  • 細胞信息: GeLu
  • 規(guī)格:frozen
  • 用途:ATCC原裝細胞
  • 服務費用:
    加載中……
  • 訂購
  • 注意事項:僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療,非藥用,非食用(產(chǎn)品信息以出庫為準)

器官來源:肺
細胞形態(tài):成纖維樣
數(shù)量:大量
運輸方式:凍存運輸
生長狀態(tài):貼壁生長
年限:403 days
是否是腫瘤細胞:0
物種來源:其他
ATCC Number:CCL-100?
規(guī)格:0.5mg Designations: GeLu
Depositors: ?ML Macy
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Meriones unguiculatus
Morphology:fibroblast


Source: Organ: lung
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Virus Resistance:poliovirus 1, 3
Age: 403 days
Gender: female
Propagation: ATCC complete growth medium: Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, rinse cell sheet twice with 0.25% trypsin - 0.53 mM EDTA solution and remove. Place culture at room temperature until cells detach (approximately 10 to 20 minutes). Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
References: 26276: . . TCA (Tissue Cult. Assoc.) Man. 3: 617-621, 1977.

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