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平臺(tái)編號(hào)：bio-68786
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國(guó)際編號(hào)：TIB-145?
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細(xì)胞信息： RA3-2C2/1
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
RA3-2C2/1
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgM
數(shù)量：大量
運(yùn)輸方式：凍存運(yùn)輸
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
ATCC Number：TIB-145?
是否是腫瘤細(xì)胞：0
物種來(lái)源：大鼠
規(guī)格：0.5ml Designations: RA3-2C2/1
Depositors: ?RL Coffman
Isotype: IgM
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against mouse B cell antigen (p50, 50000 dalton)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with the mouse cell line RAW 112.
Spleen cells were fused S194/5.XX0.BU.1 myeloma cells.
The antibody reacts with an antigen on normal B lymphocytes, a fraction of sIg- bone marrow cells and most plasma cells.
It precipitates a 50000 dalton (p50) molecule from bone marrow, spleen, thymus and Abelson leukemia virus transformed cells.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 3 to 4 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml ay maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
References: 925: Coffman RL, Weissman IL. A monoclonal antibody that recognizes B cells and B cell precursors in mice. J. Exp. Med. 153: 269-279, 1981. PubMed: 6787164
926: Rotter V, et al. Abelson murine leukemia virus-induced tumors elicit antibodies against a host cell protein, P50. J. Virol. 36: 547-555, 1980. PubMed: 6159484
免疫類型：IgM
數(shù)量：大量
運(yùn)輸方式：凍存運(yùn)輸
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
ATCC Number：TIB-145?
是否是腫瘤細(xì)胞：0
物種來(lái)源：大鼠
規(guī)格：0.5ml Designations: RA3-2C2/1
Depositors: ?RL Coffman
Isotype: IgM
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against mouse B cell antigen (p50, 50000 dalton)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with the mouse cell line RAW 112.
Spleen cells were fused S194/5.XX0.BU.1 myeloma cells.
The antibody reacts with an antigen on normal B lymphocytes, a fraction of sIg- bone marrow cells and most plasma cells.
It precipitates a 50000 dalton (p50) molecule from bone marrow, spleen, thymus and Abelson leukemia virus transformed cells.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 3 to 4 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml ay maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
References: 925: Coffman RL, Weissman IL. A monoclonal antibody that recognizes B cells and B cell precursors in mice. J. Exp. Med. 153: 269-279, 1981. PubMed: 6787164
926: Rotter V, et al. Abelson murine leukemia virus-induced tumors elicit antibodies against a host cell protein, P50. J. Virol. 36: 547-555, 1980. PubMed: 6159484
