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平臺編號：bio-68705
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國際編號：CRL-2405?
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細胞信息： SH-34
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規(guī)格：frozen
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用途：ATCC原裝細胞
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
SH-34
運輸方式：凍存運輸
ATCC Number：CRL-2405?
是否是腫瘤細胞：0
物種來源：小鼠
數(shù)量：大量
生長狀態(tài)：懸浮生長
細胞形態(tài)：淋巴樣
免疫類型：IgM
規(guī)格：1mg Designations: SH-34
Depositors: ?TJ Higgins
Isotype: IgM
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against asialo-GM1
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with the glycolipid asialo GM1 adsorbed to naked Salmonella.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody is specific for asialo-GM1. This antibody recognizes and kills by complement lysis cells known to express asialo GM1.
Most natural killer (NK) cells and cytotoxic T cells (CTL), as well as a proportion of other myeloid and lymphoid cells express asialo GM1.
The SH-34 antibody specifically lyses asialo GM1-expressing macrophages in the presence of complement and removes NK cells in vitro from spleen cell populations. [38640 ]
This monoclonal antibody can be used for the study of murine and rat hematopoietic and lymphopoietic cell lineages.
Propagation: ATCC complete growth medium: Modified Dulbecco's medium, 95%; fetal bovine serum, 5%
Temperature: 37.0°C
Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Preservation: Culture medium, 85%; fetal bovine serum, 10%; DMSO, 5%
Related Products:recommended serum:ATCC 30-2020
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
References: 38639: Stout RD, et al. Evidence that expression of asialo-GM1 may be associated with cell activation. Correlation of asialo-GM1 expression with increased total cellular RNA and protein content in normal thymocyte and spleen cell populations. J. Immunol. 139: 2123-2129, 1987. PubMed: 2443562
38640: Solomon FR, Higgins TJ. A monoclonal antibody with reactivity to asialo GM1 and murine natural killer cells. Mol. Immunol. 24: 57-65, 1987. PubMed: 3614206
38642: Suttles J, et al. Flow cytometric analysis reveals the presence of asialo GM1 on the surface membrane of alloimmune cytotoxic T lymphocytes. J. Immunol. 136: 1586-1591, 1986. PubMed: 2936802
ATCC Number：CRL-2405?
是否是腫瘤細胞：0
物種來源：小鼠
數(shù)量：大量
生長狀態(tài)：懸浮生長
細胞形態(tài)：淋巴樣
免疫類型：IgM
規(guī)格：1mg Designations: SH-34
Depositors: ?TJ Higgins
Isotype: IgM
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against asialo-GM1
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with the glycolipid asialo GM1 adsorbed to naked Salmonella.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody is specific for asialo-GM1. This antibody recognizes and kills by complement lysis cells known to express asialo GM1.
Most natural killer (NK) cells and cytotoxic T cells (CTL), as well as a proportion of other myeloid and lymphoid cells express asialo GM1.
The SH-34 antibody specifically lyses asialo GM1-expressing macrophages in the presence of complement and removes NK cells in vitro from spleen cell populations. [38640 ]
This monoclonal antibody can be used for the study of murine and rat hematopoietic and lymphopoietic cell lineages.
Propagation: ATCC complete growth medium: Modified Dulbecco's medium, 95%; fetal bovine serum, 5%
Temperature: 37.0°C
Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Preservation: Culture medium, 85%; fetal bovine serum, 10%; DMSO, 5%
Related Products:recommended serum:ATCC 30-2020
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
References: 38639: Stout RD, et al. Evidence that expression of asialo-GM1 may be associated with cell activation. Correlation of asialo-GM1 expression with increased total cellular RNA and protein content in normal thymocyte and spleen cell populations. J. Immunol. 139: 2123-2129, 1987. PubMed: 2443562
38640: Solomon FR, Higgins TJ. A monoclonal antibody with reactivity to asialo GM1 and murine natural killer cells. Mol. Immunol. 24: 57-65, 1987. PubMed: 3614206
38642: Suttles J, et al. Flow cytometric analysis reveals the presence of asialo GM1 on the surface membrane of alloimmune cytotoxic T lymphocytes. J. Immunol. 136: 1586-1591, 1986. PubMed: 2936802
